Tomivosertib

Inhibitor of MKNK1, MKNK2

Structure

Information

  • MKNK1
  • MKNK2
  • Inhibitor
  • up to 1 uM

In Vitro Validations

Uniprot ID: Q9BUB5
Target Class: Kinase
Target SubClass: CAMK
Potency: IC 50
Potency Value: 2.4 nM
Potency Assay: ADP-Glo kinase assay
PDB ID for probe-target interaction (3D structure): --
Structure-activity relationship: yes
Target aliases:
MAP kinase-interacting serine/threonine-protein ki ...

DOI Reference: 10.1021/acs.jmedchem.7b01795

In Cell Validations

In Vivo Data

Off-Target Selectivity Assesments

Potency end-point : IC50 STK17A 130 nM, CLK4 790 nM
Probe Selectivity in Vitro:

Profiled for kinase selectivity at 1 uM over 400 kinases showing only 2 off-targets

Probe Selectivity in Cell:

Tomivosertib did not show antiproliferative activity in a panel of solid and hematological tumor cell lines (IC50 > 30 μM), modest sensitivity (IC50 < 10 μM) was observed in a subset of DLBCL and a multiple myeloma cell line.

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SERP ratings and comments


SERP Ratings

In Cell Rating
In Model Organisms

SERP Comments:

eFT508 / Tomivosertib is a well characterized balanced single digit nanomolar inhibitor of MKNK1 (= MNK1) and MKNK2 (= MNK2). High selectivity was proven in a kinome selectivity screen at 1 µM compound concentration (with CLK4 and DRAK1 as the only off-targets). Cellular and in vivo target engagement was proven by dose-dependent inhibition of the specific downstream phosphorylation of elF4E. In addition, dose-dependent reduction of IL-6, IL-8 and TNFalpha was observed in TMD8 cells. It is important to note that inhibition of eFT508 / Tomivosertib does not result in tumor growth inhibition in vitro. The authors speculate that an intact in vivo tumor microenvironment is needed for tumor growth inhibition. Indeed, eFT508 / Tomivosertib has proven in vivo efficacy in DLBCL and solid tumor models. In line with the in vitro results inhibition of phospho-elF4E and cytokines has been also demonstrated in vivo. PK/PD studies lead to the conclusion that at 1mg/kg dose QD (po) produced maximal efficacy and exhibited over 80% reduction in phosho-elF4E for 8 hours. Finally, a co-crystal structure with MNK2 is available.

(last updated: 6 Sept 2024 )