THZ-P1-2 | THZ-P1-2 : Covalent Inhibitor of PIP4K2A, PIP4K2B, PIP4K2C
RATINGS:
Cellular Use: (0 reviews)

In Model Organisms: (0 reviews)
In Vivo
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
PIP4K2A
  • IC50:190 nM
  • Activity:0.2 µM
  • Activity:500 nM
  • IC50:870 - 3950 nM
  • ΔTm:9 K
PIP4K2B
  • Activity:0.2 µM
  • ΔTm:~12 K
  • Activity:500 nM
PIP4K2C
  • Activity:0.2 µM
  • Activity:500 nM
Covalent Inhibitor
up to 10 uM

Selectivity

In Vitro Selectivity Assessment
Potency Assay Off-Target:
Kinome-wide selectivity of THZ-P1-2 was assessed in the commercially available DiscoverX KINOMEscan ...
Selectivity Assessment Description:
IC50 of 40 nM on PIKfyve in a fixed time point ADP-Glo assay. The additional potential off-targets i ...
In Cell Selectivity Assessment
Selectivity Assessment Description:
To further assess if THZ-P1-2 biochemical inhibition was maintained in cells, dtb-THZ-P1-2 binding o ...

Potency
Cellular
In Vitro

PIP4K2A

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1016/j.chembiol.2020.02.003

PIP4K2B

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1016/j.chembiol.2020.02.003

PIP4K2C

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1016/j.chembiol.2020.02.003

Negative Control Compounds

THZ-P1-2-R
SMILES: CN(C)CCCC(NC1=CC=C(C=C1)C(NC2=CC=CC(NC3=CC(C4=CNC5=CC=CC=C45)=NC=N3)=C2)=O)=O

Chemical Information

Molecular Formula C31H29N7O2
SMILEs CN(C)C/C=C/C(=O)Nc1ccc(C(=O)Nc2cccc(Nc3cc(-c4c[nH]c5ccccc45)ncn3)c2)cc1
InChI InChI=1S/C31H29N7O2/c1-38(2)16-6-11-30(39)36-22-14-12-21(13-15-22)31(40)37-24-8-5-7-23(17-24)35-29-18-28(33-20-34-29)26-19-32-27-10-4-3-9-25(26)27/h3-15,17-20,32H,16H2,1-2H3,(H,36,39)(H,37,40)(H,33,34,35)/b11-6+
Molecular weight 531.24 Da
AlogP 5.677100000000004
HBond acceptors 9
HBond donors 4
Atoms 69

References

Expert Reviews


No SERP comments found for THZ-P1-2

Probe THZ-P1-2 is in the process of SERP review.

Please continue to check back for new reviews and commentary.