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T-50301 | T-50301 : Inhibitor of USP25, USP28
RATINGS:
Cellular Use: (0 reviews)

In Model Organisms: (0 reviews)
Probe Summary
Selectivity
Potency
In Vivo
Control Compounds
Chemical Information
References
Vendors
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Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
USP25
  • IC50:30 nM
  • ΔTm:7.0 K
  • Kd:200 nM
  • IC50:43 nM
  • IC50:40 nM
USP28
  • IC50:120 nM
  • ΔTm:3.8 K
  • Kd:60 nM
  • IC50:158 nM
  • IC50:60 nM
up to 1 uM

Selectivity

In Cell Selectivity Assessment
Potency Assay Off-Target:
Selectivity over other cysteine-DUB family members was assessed via Western Blot of HeLa cells.

Potency
Cellular
In Vitro

USP25

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.5c03045

USP28

Mode of Action: Inhibitor

Structure-Activity-Relationship data available? Yes

DOI Reference: 10.1021/acs.jmedchem.5c03045

Negative Control Compounds

T-10507
Notes: Cpd 33 (T-10507) had no significant binding in the ITC assay and was found to be inactive in the cellular assay.

Orthogonal Probes def

FT206

Chemical Information

Molecular Formula C26H22BrClF4N2O5S
SMILEs CS(=O)(=O)c1ccc(C(=O)Nc2cc(F)c(F)c(COc3cc(F)c(Br)cc3CN3CC[C@H](O)C3)c2F)c(Cl)c1
InChI InChI=1S/C26H22BrClF4N2O5S/c1-40(37,38)15-2-3-16(19(28)7-15)26(36)33-22-8-21(30)24(31)17(25(22)32)12-39-23-9-20(29)18(27)6-13(23)10-34-5-4-14(35)11-34/h2-3,6-9,14,35H,4-5,10-12H2,1H3,(H,33,36)/t14-/m0/s1
Molecular weight 664.01 Da
AlogP 5.4603000000000055
HBond acceptors 7
HBond donors 2
Atoms 62

Expert Reviews

No SERP comments found for T-50301

Probe T-50301 is in the process of SERP review.

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