PFI-1

Reagent Authentication Certificate:

Chemical Probes Portal PFI-1.pdf

Protein target name:

BRD2, BRD3, BRD4, BRDT

Mechanism of action:

Antagonist

Recommended Concentration for use in cells:

100 nM - 10 uM

Probe Availability

MedChem Express (1403764-72-6)

MilliporeSigma (1403764-72-6)

Tocris (1403764-72-6)

Cayman (1403764-72-6)

Probe Information

Target and Probe Information

Target Details

Target class:

Epigenetics

Subclass:

Bromodomain

HUGO ID:

HGNC:1103 HGNC:1104 HGNC:13575 HGNC:1105

Entrez gene ID:

6046 8019 23476 676

UNIPROT ID:

P25440 O60885 Q15059 Q58F21

Target alias:

D6S113E, FSH, FSRG1, NAT, O27.1.1, RING3, RNF3, ORFX, RING3L, CAP, HUNK1, HUNKI, MCAP, BRD6, CT9

Probe Details

Chemical Probes Portal ID:

CPP37

Probe alias:

QCR-192

PubChem CID:

71271629

ChEMBL ID:

CHEMBL2179387

SMILES string:

CN1CC2=C(C=CC(=C2)NS(=O)(=O)C3=CC=CC=C3OC)NC1=O

InChi Key:

TXZPMHLMPKIUGK-UHFFFAOYSA-N

PAINs evaluation:

PAINs free

Physico-chemical properties

Solubility - preferred solvent:

DMSO

Solubility - concentration:

10 mM

Storage Recommendations

Storage (dry powder):

2 years -20 oC

Storage sensitivities:

Avoid freeze-thaw

Validation in Cells and Model Organisms

In vitro validation

On target activity

Potency:

BRD4 (1) 47.4 nM, (2) 194.6 nM

Potency assay:

KD: BRD2 (1) 107.9 nM (2) 143.9 nM; BRD3 (1) 80 nM (2) 76.3 nM; BRDT (1) 85.5 nM, (2) 220 nM in ITC assays IC50 BRD2 98 nM, BRD4 220 nM in alpha screen assays

PDB ID for probe-target interaction (3D structure):

4E96

Off target activity

Potency assay (off target):

PFI-1 had negligible activity against 42 bromodomains in thermal shift assays, having the most activity against CBP/EP300, which was too weak to quantify in an IC50 assay, and making PFI-1 >300-fold selective for on-target bromodomains.

Probe Selectivity in Vitro:

PFI-1 had negligible activity against 38 protein kinases, 40 human kinases and 14 human membrane receptors (Cerep).

In cell validation

On target activity in cells

Potency assay (cells):

In U2OS cells, PFI-1 was active in a FRAP assay at 1-5 uM.

Target engagement assay (cells):

Indirect: FRAP assay

Primary Reference

Reference (doi):

10.1158/0008-5472.CAN-12-3292

Reference (PMID):

23576556

External Resources

Available data:

GDSC1

PFI-1

Probe Availability

Probe Information

Target Details

D6S113E, FSH, FSRG1, NAT, O27.1.1, RING3, RNF3, ORFX, RING3L, CAP, HUNK1, HUNKI, MCAP, BRD6, CT9

Probe Details

Physico-chemical properties

In vitro validation

In cell validation

Primary Reference

External Resources

Supporting Organizations

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PFI-1

Probe Availability

Probe Information

Target Details

D6S113E, FSH, FSRG1, NAT, O27.1.1, RING3, RNF3, ORFX, RING3L, CAP, HUNK1, HUNKI, MCAP, BRD6, CT9

Probe Details

Physico-chemical properties

In vitro validation

In cell validation

Primary Reference

External Resources

Supporting Organizations

Interested in supporting the portal?