KHK-IN-1

XNB : Inhibitor of KHK

Structure

SERP Rating

In Cells

(1 ratings)

In Model Organisms

(1 ratings)

note: The Chemical Probes Portal only endorses compounds as chemical probes for use as specific and selective modulators of the proposed target if they receive three or more (3-4) stars.

Probe KHK-IN-1 is in the process of SERP review.

Please continue to check back for new reviews and commentary.

Information

Inhibitor

up to 5 uM

Probe Availability:

MedChemExpress

In Vitro Validations

Uniprot ID: P50053

Target Class: Kinase

Target SubClass: Carbohydrate kinase

Potency: IC50

Potency Value: 12 nM

Potency Assay: Inhibition of recombinant human hepatic KHK (KHK-C) Fluorescence Polarization (FP) Assay

PDB ID for probe-target interaction (3D structure): 3Q92

Target aliases:

Ketohexokinase, KHK, KHK_HUMAN, Hepatic fructokina ...

DOI Reference: 10.1021/ml200070g

In Cell Validations

In Vivo Data

Off-Target Selectivity Assesments

Potency assay (off target): Examined in a diverse panel of 31 protein kinases, representing different families, for off-target kinase inhibition at a concentration of 10 μM (Invitrogen; with 100 μM ATP). None of the 31 kinases was inhibited >40% at this high concentration. For three off-target metabolic kinases, ribokinase, hexokinase, and adenosine kinase, the selectivity of 8 was found to be at least 50-fold. Checked against Receptors and Ion Channels at 8 and 10 uM by CEREP. All the hits were checked in biochemical assays, closest off target was norepinephrine transporter binding: IC50 = 0.83 μM

I have extra information to add

SERP ratings and comments

SERP Ratings

In Cell Rating

In Model Organisms

SERP Comments:

As well as this probe, PF-06835919 (https://doi.org/10.1021/acs.jmedchem.0c0094) which has been in clinical trials is a good probe for inhibiting KHK. Both could be used.

(last updated: 10 Oct 2023 )