JZ128 | JZ128 : Covalent Inhibitor of PKN3
RATINGS:
Cellular Use: (1 reviews)

In Model Organisms: (0 reviews)
In Vivo
Vendors

Probe Summary

Targets Biochemical/Biophysical Potency Cellular Potency
PKN3
  • IC50:120 nM
  • IC50:~100 nM
Covalent Inhibitor
up to 1 uM

Selectivity

In Cell Selectivity Assessment
Potency Assay Off-Target:
Proteome-wide selectivity of JZ128 was assessed in PC3 cells.
Selectivity Assessment Description:
Quantitative, dose-dependent competitive binding data for 686 reproducible JZ128-DTB-labeled cystein ...
In Vitro Selectivity Assessment
Potency Assay Off-Target:
In vitro kinase assays showed JZ128 to have a roughly 200 nM IC50 against SRC and RIPK2.
In Cell Selectivity Assessment
Potency Assay Off-Target:
KiNativ platform was used to interrogate JZ128 with respect to kinase inhibition.
Selectivity Assessment Description:
In total 292 kinases were detected in PC3 cells, with only three showing greater than 50% inhibition ...

Potency
Cellular
In Vitro

PKN3

Mode of Action: Covalent Inhibitor

Structure-Activity-Relationship data available? No

DOI Reference: 10.1021/jacs.8b07911

Negative Control Compounds

JZ128-R
SMILES: CC1=CC=CC2=C1C(C3=NC(NC4=CC(NC(C5=CC=C(NC(CCCN(C)C)=O)C=C5)=O)=CC=C4)=NC=C3)=CN2

Chemical Information

Molecular Formula C32H31N7O2
SMILEs Cc1cccc2[nH]cc(-c3ccnc(Nc4cccc(NC(=O)c5ccc(NC(=O)/C=C/CN(C)C)cc5)c4)n3)c12
InChI InChI=1S/C32H31N7O2/c1-21-7-4-10-28-30(21)26(20-34-28)27-16-17-33-32(38-27)37-25-9-5-8-24(19-25)36-31(41)22-12-14-23(15-13-22)35-29(40)11-6-18-39(2)3/h4-17,19-20,34H,18H2,1-3H3,(H,35,40)(H,36,41)(H,33,37,38)/b11-6+
Molecular weight 545.25 Da
AlogP 5.985520000000004
HBond acceptors 9
HBond donors 4
Atoms 72

Expert Reviews


(on 9 Jul 2026)
Cellular Use Rating
This covalent probe molecule demonstrates target engagement at <1 uM, and selective labelling of C840 of PKN3. However, off-target activity at these concentrations limits its utility as a PKN3 probe. JZ128...
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