CCT251236

CCT251236 : pirin ligand of PIR

Structure

Information

Protein target names: PIR

Mechanism of action: pirin ligand

Recommended in-cell concentration:
10-100 nM

In Vitro Validations

Uniprot ID: O00625
Target Class: Other
Target SubClass: Cupin
Potency: KD
Potency Value: 44 nM
Potency Assay: Surface Plasmon Resonance
PDB ID for probe-target interaction (3D structure): 5JCT
Structure-activity relationship: SAR for 8 analogues are available as affinities measured by SPR. The SAR is consistent with the binding mode described in by the crystal structure.
Target aliases:
Pirin, PIR, PIR_HUMAN, Probable quercetinase, Prob ...

DOI Reference: 10.1021/acs.jmedchem.6b01055

In Cell Validations

In Vivo Data

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SERP ratings and comments


SERP Ratings

In Cell Rating
In Model Organisms

SERP Comments:

This is a useful probe for Pirin - useful to study Pirin function both in cells and in vivo. There is a correlation of molecule binding to Pirin and cellular activity. Active and inactive versions of the molecule have been reported, and the inactive version should be used as a control.

(last updated: 26 Mar 2017 )

SERP Ratings

In Cell Rating
In Model Organisms

SERP Comments:

CCT251236 has been thoroughly investigated and characterized as a cellular probe for Pirin (target) and HSF (pathway) and is supported by a robust and transparent set of data. My only concern is for possible in vivo use where there is an apparent non-linear pharcacokinetic response in mouse (20 mg/kg, oral dose gave a 3-fold lower AUC than a 5 mg/kg dose, albeit in a different mouse strain). I suggest users run a pilot PK study in their strain of interest to ensure the required exposures can be achieved with their dose.

(last updated: 12 Jun 2017 )

SERP Ratings

In Cell Rating
In Model Organisms

SERP Comments:

While CCT251236 clearly shows potent binding (SPR) to human pirin, displays potent inhibition of Hsp72 induction in a cell-based assay (ELISA), and shows activity in an SK-OV-3 mouse xenograft study, this chemical probe may well be active against other targets, since the anti-proliferative activity against a panel of cancer cell lines is often more potent than the potency measured by the assays outlined above.  The enduser should thus use caution when interpreting cell-based or in vivo results obtained using CCT251236.

(last updated: 12 Mar 2021 )