BAY-299

Antagonist of BRD1, TAF1, TAF1L

Structure

Information

Protein target names: BRD1, TAF1, TAF1L

Mechanism of action: Antagonist

Recommended in-cell concentration:
2-500 nM

In Vitro Validations

Uniprot ID: O95696
Target Class: Epigenetic
Target SubClass: Bromodomain
Potency: IC 50
Potency Value: 67 nM
Potency Assay: Time-resolved fluorescence resonance energy transfer (TR-FRET) assay
PDB ID for probe-target interaction (3D structure): 5N49
Structure-activity relationship: The primary publication lists SAR of position 6, 5', and 6' of the 1,3-dimethyl-benzimidazolone core and naphthalimide moiety, respectively. Position 6 SAR was done to optimize for BRPF2 BD, BRPF1 BD, TAF1 BD2 and BRD4 BD1 activity while position 5' and 6' were used to optimize BRPF2 BD, BRPF1 BD, TAF1 BD2 and BRD4 BD1 activity. Another SAR was done to improve solubility. TF-FRET was used to generate all SAR.
Target aliases:
Bromodomain-containing protein 1, BRPF2, BRL, BRD1 ...

DOI Reference: 10.1021/acs.jmedchem.7b00306

In Cell Validations

In Vivo Data

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SERP ratings and comments


SERP Ratings

In Cell Rating
In Model Organisms

SERP Comments:

As the compound showed similar binding affinity between two type of Bromo domains (BDs) from two proteins with very different roles in transcriptional regulation means that it is not amenable for use as a chemical probe to investigate the functions of these types of BDs.

(last updated: 21 Jun 2017 )

SERP Ratings

In Cell Rating
In Model Organisms

SERP Comments:

Cellular data show activity of BAY-299 on BRPF2 BD/H3.3, BRPF2 BD/H4, TAF1 BD2/H3.3, and TAF1 BD2/H4. However, little or no anti-proliferative affect was detected.

(last updated: 30 Jun 2017 )

SERP Ratings

In Cell Rating
In Model Organisms

SERP Comments:

Because the compound showed similar binding affinity for two types of Bromodomains (BDs) from two proteins with very different roles in transcriptional regulation, it is not amenable for use as a chemical probe to investigate the functions of these types of BDs.

(last updated: 28 Jul 2017 )